• 云顶集团3118(中国)

    Visium Direct Mount FFPE v2 -- University of Minnesota TMCs

    Laura J Niedernhofer, David A Bernlohr

    Published: 2023-12-21 DOI: 10.17504/protocols.io.kxygx3qpkg8j/v2
    AI 解读
    浏览 1501

    Abstract

    The Visium Spatial Gene Expression for FFPE is designed to measure mRNA in tissue sections derived

    from formalin fixed & paraffin embedded (FFPE) tissue samples and requires a Visium Spatial slide

    with intact tissue sections as input.

    Tissue sections are processed with Visium Spatial Gene Expression assay performed by the University of Minnesota Imaging Center and sequenced by the University of Minnesota Genomics Center.

    Steps

    Deparaffinization, H&E Staining, Imaging & Decrosslinking

    1.

    CG000409_VisiumSpatialFFPE_Deparaffin_H_E_RevC.pdf

    Library Preparation & Sequencing

    2.

    CG000407_VisiumSpatialGeneExpressionforFFPE_UserGuide_RevD.pdf

    Note
    Sequencer: Illumina NovaSeq 6000 using read format 28/10/10/50

    FASTQ Generation

    3.

    BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq software version 2.20.0

    推荐阅读

    Nature Protocols
    Protocols IO
    Current Protocols
    扫码咨询