Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases

Kuok Yap, Junqiao Du, Fabian B. H. Rehm, Shyn Ric Tang, Yan Zhou, Jing Xie, Conan K. Wang, Simon J. de Veer, Linda H. L. Lua, Thomas Durek, David J. Craik

Published: 2021-02-16 DOI: 10.1038/s41596-020-00483-0

Pichia pastoris

cyclic peptides

asparaginyl endopeptidases

bioproduction

enzymatic cyclization

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Cyclic disulfide-rich peptides have attracted significant interest in drug development and biotechnology. Here, we describe a protocol for producing cyclic peptide precursors in Pichia pastoris that undergo invitro enzymatic maturation into cyclic peptid
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ACKNOWLEDGMENTS

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Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases

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