Tutorial: best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation

Ernesto S. Nakayasu, Marina Gritsenko, Paul D. Piehowski, Yuqian Gao, Daniel J. Orton, Athena A. Schepmoes, Thomas L. Fillmore, Brigitte I. Frohnert, Marian Rewers, Jeffrey P. Krischer, Charles Ansong, Astrid M. Suchy-Dicey, Carmella Evans-Molina, Wei-Jun Qian, Bobbie-Jo M. Webb-Robertson, Thomas O. Metz

Published: 2021-07-08 DOI: 10.1038/s41596-021-00566-6

mass spectrometry

protein biomarkers

study design

data processing

statistical analysis

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Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

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Tutorial: best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation

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