This collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way. This collection follows nucleofection of hPSCs as described in detail in the collection "Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs;" dx.doi.org/10.17504/protocols.io.b4qnqvve

Collection overview

Seeding nucleofected hPSCs in 96-well plates using limited dilution

Duplicating 96-well plate-cultured hPSCs clones

Subcloning of genotype-confirmed hPSCs clones

General notes

1. Throughout these protocols, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.