Stable isotope tracing to assess tumor metabolism in vivo

Brandon Faubert, Alpaslan Tasdogan, Sean J. Morrison, Thomas P. Mathews, Ralph J. DeBerardinis

Published: 2021-09-16 DOI: 10.1038/s41596-021-00605-2

mass spectrometry

stable isotope tracing

tumor metabolism

in vivo analysis

[13C]glucose infusions

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Cancer cells undergo diverse metabolic adaptations to meet the energetic demands imposed by dysregulated growth and proliferation. Assessing metabolism in intact tumors allows the investigator to observe the combined metabolic effects of numerous cancer c
Introduction
Materials
Procedure 1: clinical infusions
Procedure 2: mouse infusion
Procedure 3: downstream analysis
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Stable isotope tracing to assess tumor metabolism in vivo

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