Take the medium in powdered form and mix it with doubly distilled water to just 1/10 th of the total volume.

Add a common stock of micronutrients made in ddH2O and mix well.

Make up the volume to the total and mixed well.

Adjust the pH on a calibrated pH meter using 1N HCl and /or 1N NaOH. The media was mixed using a magnetic stirrer continuously to homogenize the pH and to prevent precipitation of certain constituents.

This mix was autoclaved.