Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection

Rajendra Kumar Gurumurthy, Stefanie Koster, Naveen Kumar, Thomas F. Meyer, Cindrilla Chumduri

Published: 2022-05-10 DOI: 10.1038/s41596-022-00695-6

Organoids

Cervical Cancer

Genetic Manipulation

Infection Modeling

Stem Cells

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Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Chumduri, C. & Turco, M. Y. Organoids of the female reproductive tract. J. Mol. Med. 99, 531–553 (2021).
Wira, C. R., Rodriguez-Garcia, M. & Patel, M. V. The role of sex hormones in immune protection of the female reproductive tract. Nat. Rev. Immunol. 15, 217–230 (2015).
Wira, C. R., Grant-Tschudy, K. S. & Crane-Godreau, M. A. Epithelial cells in the female reproductive tract: a central role as sentinels of immune protection. Am. J. Reprod. Immunol. 53, 65–76 (2005).
Chumduri, C. et al. Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nat. Cell Biol. 23, 184–197 (2021).
Martyn, F., McAuliffe, F. M. & Wingfield, M. The role of the cervix in fertility: is it time for a reappraisal? Hum. Reprod. 29, 2092–2098 (2014).
WCRF (2018).
Chan, C. K., Aimagambetova, G., Ukybassova, T., Kongrtay, K. & Azizan, A. Human papillomavirus infection and cervical cancer: epidemiology, screening, and vaccination-review of current perspectives. J. Oncol. 2019, 3257939 (2019).
Zhu, H., Shen, Z., Luo, H., Zhang, W. & Zhu, X. Chlamydia trachomatis infection-associated risk of cervical cancer: a meta-analysis. Medicine 95, e3077 (2016).
Shen, R., Richter, H. E. & Smith, P. D. Interactions between HIV-1 and mucosal cells in the female reproductive tract. Am. J. Reprod. Immunol. 71, 608–617 (2014).
WHO (2018).
Schutgens, F. & Clevers, H. Human organoids: tools for understanding biology and treating diseases. Annu. Rev. Pathol. 15, 211–234 (2020).
Kim, J., Koo, B. K. & Knoblich, J. A. Human organoids: model systems for human biology and medicine. Nat. Rev. Mol. Cell Biol. 21, 571–584 (2020).
Koster, S. et al. Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogramming. Nat. Commun. 13, 1030 (2022).
Clevers, H., Loh, K. M. & Nusse, R. Stem cell signaling. An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control. Science 346, 1248012 (2014).
Hsu, Y. C., Li, L. & Fuchs, E. Transit-amplifying cells orchestrate stem cell activity and tissue regeneration. Cell 157, 935–949 (2014).
Huch, M. et al. Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell 160, 299–312 (2015).
Rheinwald, J. G. & Green, H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 265, 421–424 (1977).
Llames, S., García-Pérez, E., Meana, Á., Larcher, F. & del Río, M. Feeder layer cell actions and applications. Tissue Eng. B 21, 345–353 (2015).
Herfs, M. et al. A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer. Proc. Natl Acad. Sci. USA 109, 10516–10521 (2012).
Koskela, P. et al. Chlamydia trachomatis infection as a risk factor for invasive cervical cancer. Int. J. Cancer 85, 35–39 (2000).
Ssedyabane, F. et al. HPV-chlamydial coinfection, prevalence, and association with cervical intraepithelial lesions: a pilot study at Mbarara Regional Referral Hospital. J. Cancer Epidemiol. 2019, 9092565 (2019).
Lohmussaar, K. et al. Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. Cell Stem Cell 28, 1380–1396 (2021).
Hoffmann, K. et al. Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment. EMBO J. 39, e104013 (2020).
Lohmussaar, K. et al. Assessing the origin of high-grade serous ovarian cancer using CRISPR-modification of mouse organoids. Nat. Commun. 11, 2660 (2020).
Dolat, L. & Valdivia, R.H. An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells. J. Cell Sci. 134, jcs252403 (2021).
Kessler, M. et al. Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat. Commun. 10, 1194 (2019).
Fatehullah, A., Tan, S. H. & Barker, N. Organoids as an in vitro model of human development and disease. Nat. Cell Biol. 18, 246–254 (2016).
Sato, T. et al. Single Lgr5 stem cells build crypt–villus structures in vitro without a mesenchymal niche. Nature 459, 262–265 (2009).
Katz, D. F. Human cervical mucus: research update. Am. J. Obstet. Gynecol. 165, 1984–1986 (1991).
Merbah, M. et al. Cervico-vaginal tissue ex vivo as a model to study early events in HIV-1 infection. Am. J. Reprod. Immunol. 65, 268–278 (2011).
Michelini, M., Rosellini, A., Simoncini, T., Papini, S. & Revoltella, R. P. A three-dimensional organotypic culture of the human uterine exocervix for studying mucosal epithelial differentiation and migrating leukocytes. Differentiation 72, 138–149 (2004).
Cattaneo, C. M. et al. Tumor organoid-T-cell coculture systems. Nat. Protoc. 15, 15–39 (2020).
Zadora, P. K. et al. Integrated phosphoproteome and transcriptome analysis reveals chlamydia-induced epithelial-to-mesenchymal transition in host cells. Cell Rep. 26, 1286–1302 (2019).
Farin, H. F., Van, Es,J. H. & Clevers, H. Redundant sources of Wnt regulate intestinal stem cells and promote formation of Paneth cells. Gastroenterology 143, 1518–152s (2012).
Willert, K. et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423, 448–452 (2003).
Gurumurthy, R. K. et al. A loss-of-function screen reveals Ras-and Raf-independent MEK–ERK signaling during Chlamydia trachomatis infection. Sci. Signal. 3, ra21 (2010).
Fuerer, C. & Nusse, R. Lentiviral vectors to probe and manipulate the Wnt signaling pathway. PLoS One 5, e9370 (2010).

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection

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