10% Triton X-100 (100mL)

A	B	C	D
Solution	Final Conc	Volume	Notes
Triton X-100	10%	10 mL
Ultrapure Water		Fill to: 100 mL

1Molarity (M) MgCl₂ (100mL)

A	B	C	D
Solution	Final Conc	Volume	Notes
MgCl2 (FW 203.31)	1 M	20.331 g	203.31 g/1000 mL = 1 M 5.08275 g/100 mL = 1 M
Ultrapure Water		Fill to: 100 mL

A	B	C	D
Solution	Final Conc	1 RXN	5.2 RXN
10x PBS	1x	300 uL	1560 uL
1M MgCl2	3 mM	9 uL	46.8 uL
D-Sucrose (342.29)	0.3 M	0.3081 g	1.602 g
** RNasein (add on day)	0.4 U/uL	30 uL	156 uL
Ultrapure Water		Fill to: 3 mL	Fill to: 5.6 mL

Add most of the Ultrapure water, then PBS, then MgCl₂, then Sucrose.

Vortex to dissolve.

Then add Ultrapure water to final volume.

Add RNasein before using.

A	B	C	D
Solution	Final Conc	1 RXN	5.2 RXN
10x PBS	1x	0.8 mL	4.16 mL
1 M MgCl2	3 mM	24 uL	124.8 uL
D-Sucrose	1.4 M	3.8336 g	19.935 g
10% Triton X-100	0.10%	80 uL	416 uL
Ultrapure Water		Fill to: 8 mL	Fill to: 41.6 mL

Add half of the Ultrapure water, then PBS, then MgCl₂, then Sucrose.

Vortex to dissolve.

Then add 10% Triton.

Then add Ultrapure water to final volume.

A	B	C	D
Solution	Final Conc	1 RXN	5.2 RXN
10x PBS	1x	400 uL	2080 uL
50mg/uL Ultrapure BSA	1%	800 uL (40 mg)	4160 uL
** RNasein (add on day)	0.2 U/uL	20 uL	104 uL
Ultrapure Water		Fill to: 4 mL	Fill to: 20.8 mL

Add most of the Ultrapure water, then PBS, then BSA.

Tilt to dissolve, do not vortex.

Add RNasein before using.