Genome-wide measurement of DNA replication fork directionality and quantification of DNA replication initiation and termination with Okazaki fragment sequencing

Nataliya Petryk, Xia Wu, Yaqun Liu, Yves d’Aubenton-Carafa, Claude Thermes, Olivier Hyrien, Chun-Long Chen

Published: 2023-01-17 DOI: 10.1038/s41596-022-00793-5

Okazaki fragment sequencing

DNA replication

Replication fork directionality

Genome stability

Hidden Markov model

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Studying the dynamics of genome replication in mammalian cells has been historically challenging. To reveal the of replication initiation and termination in the human genome, we developed Okazaki fragment sequencing (OK-seq), a quantitative appro
Introduction
Materials
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Timing
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Anticipated results
References
ACKNOWLEDGMENTS

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Genome-wide measurement of DNA replication fork directionality and quantification of DNA replication initiation and termination with Okazaki fragment sequencing

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