Genetic circuit design automation with Cello 2.0

Christopher A. Voigt, Timothy S. Jones, Samuel M. D. Oliveira, Chris J. Myers, Douglas Densmore

Published: 2022-02-22 DOI: 10.1038/s41596-021-00675-2

Synthetic biology

Cello 2.0

Genetic circuit design

Computer-aided design

DNA sequence automation

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Sections

Figures

References

Cells interact with their environment, communicate among themselves, track time and make decisions through functions controlled by natural regulatory genetic circuits consisting of interacting biological components. Synthetic programmable circuits used in
Introduction
Materials
- Equipment
Procedure
Timing
Troubleshooting
Anticipated results
References
ACKNOWLEDGMENTS

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Genetic circuit design automation with Cello 2.0

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