Transfer the mice as single cage into a “Green line” cage (Techniplast, USA).

Provide few pellet of food (Standard diet, SDS, R1/CRM/R3) (around 2-3 pellets for a total of 5g) as well as water ad libitum.

Place a 5 x 5 cm cellulose paper tissue folded in the right corner of the cage.

Place the cage in a ventilated rack with temperature, humidity and light control for 48h.

At the end of the 48h check the animal cage and take a picture.

Score the bedding as follow:

0. The mice did not touch the paper square.

1. The mice unfolded the paper square but did not tear it up.

2. The mice unfolded and started to tore up <10% of the paper square.

3. The mice tore up the paper square <50%

4. The mice tore up the paper square, but the bedding is not perfect.

5. Perfect bedding